C1 Organic Synthesis · C₂₆H₁₇NO₅ · MW 433.42 g/mol · Fluorescent probe

Aminophenyl Fluorescein (APF)

Cell-permeable, photostable probe for selective detection of highly reactive oxygen and nitrogen species. On reaction with hROS/hRNS it is cleaved to release free fluorescein, giving a strong signal on standard microscopes, flow cytometers and plate readers.

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Stage: Synthesis to order
Selectivity: OCl⁻ · HO· · ONOO⁻
Indicative: Price on requestby quantity and application

Chemical structure of aminophenyl fluorescein (APF) — APF — aminophenyl fluorescein, structural formula

Scientific summary

APF is a cell-permeable, photostable probe for selective detection of highly reactive oxygen and nitrogen species. On reaction with hROS/hRNS the probe is cleaved and releases free fluorescein, producing a strong fluorescence signal readable on standard fluorescence microscopes, flow cytometers and plate readers.

Selectively detects

Hypochlorite (OCl⁻)
Hydroxyl radical (HO·)
Peroxynitrite (ONOO⁻)

Minimal reactivity with

H₂O₂
O₂·⁻
NO
¹O₂
ROO·

Applications

Live-cell imaging of oxidative burst and nitrosative stress
Flow cytometry quantification of hROS/hRNS production
High-content screening for antioxidant compounds
Mechanistic studies of NF-κB, apoptosis and inflammation pathways
Antimicrobial and phagocytosis assay systems

Specification

Compound type	Fluorescein-based fluorescent probe
Molecular formula	C₂₆H₁₇NO₅
Molecular weight	433.42 g/mol
Research group	Department of Organic Chemistry, Faculty of Science, UPJŠ
Evidence status	Synthesis capability; analytical characterisation supplied with the material
Supply	Synthesised to order · custom quantities
Engagement	Supply agreement · custom synthesis · pilot
Indicative price	Price on request, by quantity and application

Supplied for laboratory research use. Also available together with NPF as a combined probe panel.

What happens next

Book a call or send an inquiry. Tell us the target, the application and the timeline.
We answer within three working days — including when the answer is that we cannot help.
NDA before disclosure, if you need one. Tick the box on the form.
Feasibility scan or pilot proposal with a defined scope, deliverable and price.

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Common questions

How does an engagement start?

With a free 30-minute discovery call. We establish whether there is a fit and what the right next step is. Nothing is committed at that point.

Can we sign an NDA before we describe our project?

Yes. Tick the NDA box on the inquiry form and we arrange it before any disclosure.

Are the prices on this site binding?

No. They are indicative ranges so that you can size a project before contacting us. Final terms are negotiated per scope.

How quickly do you reply?

Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

What happens if you cannot help?

We say so, early, and where we can we point you to someone who can. A negative feasibility answer delivered quickly is a useful result.

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Readout: Absorbance · fluorescence
Stage: Synthesis to order
Indicative: Price on requestby quantity and application

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Evidence: Synthetic capability · characterisation on request
Stage: Synthesis to order · licensing open
Indicative: Price on requestby quantity and application

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Tell us what you are trying to achieve.

A free 30-minute call establishes whether there is a fit and what the right next step is. Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

Book a 30-minute discovery call Send an inquiry instead