C2 Organic Synthesis · C₂₆H₁₅NO₇ · MW 463.40 g/mol · Reporter substrate

Nitrophenyl Fluorescein (NPF)

A substrate and reporter group for enzymatic and bioanalytical assays. Enzyme-mediated hydrolysis or reduction of the nitrophenyl ester bond changes absorbance and fluorescence, producing an optical signal proportional to enzyme activity or analyte concentration.

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Stage: Synthesis to order
Readout: Absorbance · fluorescence
Indicative: Price on requestby quantity and application

Chemical structure of nitrophenyl fluorescein (NPF) — NPF — nitrophenyl fluorescein, structural formula

Scientific summary

NPF serves as a substrate or reporter group in enzymatic and bioanalytical assay systems. A chemical transformation — typically enzyme-mediated hydrolysis or reduction of the nitrophenyl ester bond — changes the compound's absorbance and fluorescence properties, generating an optical signal proportional to enzyme activity or analyte concentration.

Primary use cases

Enzyme activity reporter
Bioanalytical assays
Esterase substrates
Hydrolase profiling

Detection methods

Absorbance (colorimetric)
Fluorescence
Plate-reader compatible

Applications

Enzymatic activity assays — esterases, lipases, hydrolases
Bioanalytical detection of specific analytes via reporter release
High-throughput screening substrates for enzyme panels
Quality control and calibration standards in optical assays
Custom reporter substrate design under NDA

Specification

Compound type	Fluorescein-based reporter substrate
Molecular formula	C₂₆H₁₅NO₇
Molecular weight	463.40 g/mol
Research group	Department of Organic Chemistry, Faculty of Science, UPJŠ
Evidence status	Synthesis capability; analytical characterisation supplied with the material
Supply	Synthesised to order · custom quantities
Engagement	Supply agreement · custom synthesis · pilot
Indicative price	Price on request, by quantity and application

What happens next

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We answer within three working days — including when the answer is that we cannot help.
NDA before disclosure, if you need one. Tick the box on the form.
Feasibility scan or pilot proposal with a defined scope, deliverable and price.

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Common questions

How does an engagement start?

With a free 30-minute discovery call. We establish whether there is a fit and what the right next step is. Nothing is committed at that point.

Can we sign an NDA before we describe our project?

Yes. Tick the NDA box on the inquiry form and we arrange it before any disclosure.

Are the prices on this site binding?

No. They are indicative ranges so that you can size a project before contacting us. Final terms are negotiated per scope.

How quickly do you reply?

Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

What happens if you cannot help?

We say so, early, and where we can we point you to someone who can. A negative feasibility answer delivered quickly is a useful result.

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Selectivity: OCl⁻ · HO· · ONOO⁻
Stage: Synthesis to order
Indicative: Price on requestby quantity and application

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Evidence: Synthetic capability · characterisation on request
Stage: Synthesis to order · licensing open
Indicative: Price on requestby quantity and application

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Evidence: Synthetic capability · characterisation on request
Stage: Synthesis to order · licensing open
Indicative: Price on requestby quantity and application

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Tell us what you are trying to achieve.

A free 30-minute call establishes whether there is a fit and what the right next step is. Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

Book a 30-minute discovery call Send an inquiry instead