B3 Proteins & Enzymes · Protein biophysics · Formulation support

Find out how your protein actually behaves — before formulation decisions become expensive to reverse.

DSC, DLS, fluorescence probes and a systematic buffer, salt and pH matrix, run on your protein rather than predicted from a general series. You receive a stability map, a ranked set of conditions and an aggregation risk profile in 4 to 8 weeks. Delivered by the Centre for Interdisciplinary Biosciences under prof. Erik Sedlák. From € 8 000.

Book a 30-minute call about your protein Request the Protein Stability technical brief

Evidence: Method portfolio · report under NDA
Stage: Pilot-ready
Indicative: € 8 000 – 50 000negotiable by scope and application

Specific-ion and Hofmeister effects on enzyme stability are this group’s published research area — see Dušeková et al., Biophysical Chemistry 2022;287:106825. The methods we would apply to your protein are the methods behind that paper.

The problem this solves

Stability problems are almost never found early. They surface during scale-up, during a stability study, or in a failed batch — at which point reformulation costs months and the comparability work starts again. The measurements that would have prevented it are neither exotic nor expensive. They are simply rarely run before the formulation space has already been narrowed by convenience.

What the platform does

It maps how your protein actually behaves before formulation decisions are locked in: thermodynamic and kinetic stability by differential scanning calorimetry and fluorescence probes, size distribution and early aggregation by dynamic light scattering, and systematic buffer, salt and pH screening. Hofmeister effects are measured on your protein rather than assumed from a general series.

Why it pays for itself

Stability problems discovered late are expensive: reformulation, repeated stability studies, lost batches. Measuring the landscape early narrows the formulation space to conditions that are already known to work.

Applications

Formulation development support
Buffer and excipient selection
Aggregation risk profiling
Comparability and stability studies

Instrumentation & readouts

Differential scanning calorimetry (DSC)
Fluorescence probe measurements
Dynamic light scattering (DLS)
Circular dichroism where relevant
Buffer / salt / pH matrix screening

Specification

Technology type	Biophysical characterisation of proteins
Research group	CIB — Centre for Interdisciplinary Biosciences
Evidence status	Pilot-ready capability; results generated for the partner under NDA
Typical pilot	Bioactivity/biophysics pilot, 4–8 weeks
Deliverables	Stability maps, condition ranking, technical report
Engagement	Feasibility scan · pilot · programme
Indicative price	€ 8 000 – 50 000, negotiable by scope and application

What happens next

Book a call or send an inquiry. Tell us the target, the application and the timeline.
We answer within three working days — including when the answer is that we cannot help.
NDA before disclosure, if you need one. Tick the box on the form.
Feasibility scan or pilot proposal with a defined scope, deliverable and price.

Book a 30-minute discovery call Send an inquiry

Common questions

How much material do you need?

The requirement depends on the assay set and the concentration your protein tolerates. TODO: confirm minimum quantity and concentration with CIB before go-live.

Do you work under NDA?

Yes. We sign before you send material or sequence information.

Who owns the results?

Ownership of project results is agreed in writing before work starts, in the pilot agreement. TODO: insert the standard UPJŠ results-ownership clause.

Can you work with a construct that is not yet expressing well?

That is a different platform — see B5, MBP fusion purification. At the call we will tell you which one you actually need.

What if the answer is that our protein is inherently unstable?

Then you have that answer in eight weeks instead of eighteen months. That is a successful pilot.

How does an engagement start?

With a free 30-minute discovery call. We establish whether there is a fit and what the right next step is. Nothing is committed at that point.

Can we sign an NDA before we describe our project?

Yes. Tick the NDA box on the inquiry form and we arrange it before any disclosure.

Are the prices on this site binding?

No. They are indicative ranges so that you can size a project before contacting us. Final terms are negotiated per scope.

How quickly do you reply?

Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

What happens if you cannot help?

We say so, early, and where we can we point you to someone who can. A negative feasibility answer delivered quickly is a useful result.

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Tell us what you are trying to achieve.

A free 30-minute call establishes whether there is a fit and what the right next step is. Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

Book a 30-minute discovery call Send an inquiry instead