B5 Proteins & Enzymes · Recombinant protein purification

MBP Fusion Protein Purification Platform

One-step affinity purification and immobilisation for MBP-tagged proteins. The MBP tag substantially improves solubility for difficult-to-express fusion partners — a cost-effective alternative for teams spending too much on multi-step chromatography of insoluble targets.

Book a 30-minute discovery call Request the data package

Evidence: Method portfolio · report under NDA
Stage: Pilot-ready
Indicative: € 5 000 – 40 000negotiable by scope and application

What the platform does

Maltose-binding protein is used here for two things at once: as a solubility enhancer for fusion partners that otherwise end up in inclusion bodies, and as the handle for one-step affinity purification and immobilisation. The workflow is designed to remove chromatography steps rather than add them.

Who benefits most

Teams whose target is expressible but not solubly expressible, and teams whose purification cost per milligram has quietly become the limiting factor in how many constructs they can test.

Applications

Difficult-to-express fusion proteins
One-step affinity purification
Protein immobilisation
Construct design support

Methods available

MBP fusion construct design
Expression and solubility screening
Affinity purification and immobilisation
Yield and purity reporting

Specification

Technology type	Fusion-tag expression and purification workflow
Research group	CIB — Centre for Interdisciplinary Biosciences
Evidence status	Pilot-ready capability; results generated for the partner under NDA
Typical pilot	4–8 weeks per construct set
Deliverables	Purified material, yield and purity data, protocol
Engagement	Feasibility scan · pilot
Indicative price	€ 5 000 – 40 000, negotiable by scope and application

What happens next

Book a call or send an inquiry. Tell us the target, the application and the timeline.
We answer within three working days — including when the answer is that we cannot help.
NDA before disclosure, if you need one. Tick the box on the form.
Feasibility scan or pilot proposal with a defined scope, deliverable and price.

Book a 30-minute discovery call Send an inquiry

Common questions

How does an engagement start?

With a free 30-minute discovery call. We establish whether there is a fit and what the right next step is. Nothing is committed at that point.

Can we sign an NDA before we describe our project?

Yes. Tick the NDA box on the inquiry form and we arrange it before any disclosure.

Are the prices on this site binding?

No. They are indicative ranges so that you can size a project before contacting us. Final terms are negotiated per scope.

How quickly do you reply?

Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

What happens if you cannot help?

We say so, early, and where we can we point you to someone who can. A negative feasibility answer delivered quickly is a useful result.

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Tell us what you are trying to achieve.

A free 30-minute call establishes whether there is a fit and what the right next step is. Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

Book a 30-minute discovery call Send an inquiry instead