B4 Proteins & Enzymes · Protease activity · Condition optimisation

HRV 3C Protease Optimisation Platform

Kosmotropic anions increase HRV 3C protease rigidity, stability and catalytic efficiency — a published finding with direct workflow consequences. If your production depends on a cleavage step, this tells you which conditions maximise yield and enzyme lifetime.

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Evidence: Peer-reviewed 2022 (Biophys. Chem.)
Stage: Pilot-ready
Indicative: € 6 000 – 35 000negotiable by scope and application

Scientific summary

Our published study tested five sodium anions — sulfate, phosphate, chloride, bromide and perchlorate — against the catalytic and conformational properties of HRV 3C protease, using circular dichroism, tyrosine fluorescence, differential scanning calorimetry and molecular dynamics simulations. In the presence of kosmotropic anions the enzyme showed increased stability and rigidity, and catalytic efficiency increased, consistent with the Hofmeister effect.

Direct workflow consequence

If tag removal by HRV 3C sits on your critical path, buffer composition is not a neutral choice. The published correlations let us screen conditions rationally instead of by trial and error, and report which combination maximises cleavage yield and enzyme lifetime for your fusion construct.

Applications

Recombinant protein production workflows
Affinity tag removal optimisation
Protease condition screening
Enzyme stability engineering

Assays & readouts available

Activity measurement across salt series
DSC thermal stability
Circular dichroism conformational analysis
Cleavage efficiency on partner constructs

Specification

Technology type	Enzyme condition optimisation (Hofmeister / specific anion effects)
Research group	CIB — Centre for Interdisciplinary Biosciences (prof. Sedlák)
Evidence status	Published evidence
Publication	Dušeková E et al., Biophysical Chemistry 2022;287:106825 — doi:10.1016/j.bpc.2022.106825
Typical pilot	4–8 weeks on the partner's construct
Deliverables	Optimal condition set, activity and stability data, protocol
Engagement	Feasibility scan · pilot
Indicative price	€ 6 000 – 35 000, negotiable by scope and application

What happens next

Book a call or send an inquiry. Tell us the target, the application and the timeline.
We answer within three working days — including when the answer is that we cannot help.
NDA before disclosure, if you need one. Tick the box on the form.
Feasibility scan or pilot proposal with a defined scope, deliverable and price.

Book a 30-minute discovery call Send an inquiry

Common questions

How does an engagement start?

With a free 30-minute discovery call. We establish whether there is a fit and what the right next step is. Nothing is committed at that point.

Can we sign an NDA before we describe our project?

Yes. Tick the NDA box on the inquiry form and we arrange it before any disclosure.

Are the prices on this site binding?

No. They are indicative ranges so that you can size a project before contacting us. Final terms are negotiated per scope.

How quickly do you reply?

Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

What happens if you cannot help?

We say so, early, and where we can we point you to someone who can. A negative feasibility answer delivered quickly is a useful result.

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Tell us what you are trying to achieve.

A free 30-minute call establishes whether there is a fit and what the right next step is. Every inquiry is acknowledged within one working day and answered by the responsible group leader within three.

Book a 30-minute discovery call Send an inquiry instead